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Protocol for sortase-mediated construction of DNA-protein hybrids and functional nanostructures.

Methods.. 2014-02; 
MA Koussa, M Sotomayor, WP Wong. Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, United States.
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摘要

Recent methods in DNA nanotechnology are enabling the creation of intricate nanostructures through the use of programmable, bottom-up self-assembly. However, structures consisting only of DNA are limited in their ability to act on other biomolecules. Proteins, on the other hand, perform a variety of functions on biological materials, but directed control of the self-assembly process remains a challenge. While DNA-protein hybrids have the potential to provide the best-of-both-worlds, they can be difficult to create as many of the conventional techniques for linking proteins to DNA render proteins dysfunctional. We present here a sortase-based protocol for covalently coupling proteins to DNA with minimal disturba... More

关键词

DNA-protein hybrids/chimeras; Molecular self-assembly; Sortase coupling; Bioconjugation; DNA origami; Site-directed