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Photoexcited Cryptochrome 1 Interacts With SPCHLESS to Regulate Stomatal Development in Arabidopsis

PLANT CELL AND ENVIRONMENT. 2024-09; 
Li Chen 1 2, Xiaoli Cao , Yupeng Li 1 2, Minqing Liu 1, Yao Liu 1, Yan Guan 1 2, Jiaqi Ruan 1, Zhilei Mao 1 2, Wenxiu Wang 1 2, Hong-Quan Yang 1 2, Tongtong Guo
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Antibody Modification and Purification Services Proteins associated with the beads were eluted into a 2 × SDS loading buffer and analysed by western blot analysis. The bait protein was visualized using Coomassie brilliant blue (CBB) staining, while the prey protein was detected using an anti‐His antibody (1:1000; GenScript, A00186). Get A Quote

摘要

Stomata are epidermal openings that facilitate plant-atmosphere gas and water exchange during photosynthesis, respiration and water evaporation. SPEECHLESS (SPCH) is a master basic helix-loop-helix (bHLH) transcription factor that determines the initiation of stomatal development. It is known that blue light promotes stomatal development through the blue light photoreceptor cryptochromes (CRYs, CRY1 and CRY2). Whether CRYs regulate stomatal development through directly modulating SPCH is unknown. Here, we demonstrate by biochemical studies that CRY1 physically interacts with SPCH in a blue light-dependent manner. Genetic studies show that SPCH acts downstream of CRY1 to promote stomatal development in blue ligh... More

关键词

SPEECHLESS (SPCH); cryptochromes; light signalling; photoreceptor; stomatal development.