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PICKLE-mediated nucleosome condensing drives H3K27me3 spreading for the inheritance of Polycomb memory during differentiation

Molecular Cell. 2024-09; 
Zhenwei Liang, ∙ Tao Zhu, ∙ Yaoguang Yu,∙ … ∙ Yuhai Cui ∙ Shangzhi Huang ∙ Chenlong Li
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Molecular Biology Reagents Proteins were loaded into 4%–10% gradient protein gels (GenScript, SurePAGE, cat. no. M00653) and electrophoresed at 150 V for 2 h. Get A Quote

摘要

Spreading of H3K27me3 is crucial for the maintenance of mitotically inheritable Polycomb-mediated chromatin silencing in animals and plants. However, how Polycomb repressive complex 2 (PRC2) accesses unmodified nucleosomes in spreading regions for spreading H3K27me3 remains unclear. Here, we show in Arabidopsis thaliana that the chromatin remodeler PICKLE (PKL) plays a specialized role in H3K27me3 spreading to safeguard cell identity during differentiation. PKL specifically localizes to H3K27me3 spreading regions but not to nucleation sites and physically associates with PRC2. Loss of PKL disrupts the occupancy of the PRC2 catalytic subunit CLF in spreading regions and leads to aberrant dedifferentiation. Nucle... More

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