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In Situ Noninvasive Monitoring of Cell Secretions Based on MOFs/AAO Hybrid Membrane Induced Asymmetric Ion Transport

Biosensors & bioelectronics. 2024-09; 
Zhaohan Wang , Yafeng Wu , Zhi Wang , Zhaoyan Tian , Yu Liu , Songqin Liu
Products/Services Used Details Operation
Recombinant Proteins TNF-α was from GenScript. Human TNF-α ELISA Kit was from Yeasen. TNF-α aptamer VR11 (5′-NH2-TGG TGG ATG GCG CAG TCG GCG ACA A-3′) was purchased from Shanghai Sangon Biotechnology Co. Ltd. Get A Quote

摘要

Nanofluidic hybrid membranes display distinct ionic current rectification (ICR) properties and provide high surface area for immobilizing probes on the outer surface, exhibiting great potential in detection of biomolecules. Herein, we fabricated MOFs/AAO hybrid membrane with aptamers functionalized on the outer surface for in situ detection of living cells released secretions. TNF-α (a small molecular protein secreted by macrophages) was used as a model. After TNF-α was specifically captured by aptamers on the membrane surface, the asymmetry of surface charge on the hybrid membrane was amplified, the ICR was increased from 3.89 to 18.85. According to the ICR change, TNF-α was sensitively measured with a dete... More

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