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Rapid detection of in sputum using CRISPR-Cas12b combined with cross-priming amplification in a single reaction

J Clin Microbiol. 2023-12; 
Lijun Peng, Tingting Fang, Qingshan Cai, Hao Li, Huanyu Li, Haiqiong Sun, Mingzhi Zhu, Lingshan Dai, Yanqin Shao, Long Cai
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Molecular Biology Reagents … The target segment of IS6110 was cloned into the pUC57 plasmid, generating a single-copy pUC-IS6110 plasmid (GenScript, China) to be used as the template. The concentration of … Get A Quote

摘要

In this study, we successfully established a new One-Pot method, named TB One-Pot, for detecting Mtb in sputum by combining CRISPR-cas12b-mediated trans-cleavage with cross-priming amplification (CPA). Our study evaluated the diagnostic performance of TB One-Pot in clinical sputum samples for tuberculosis. The findings provide evidence for the potential of TB One-Pot as a diagnostic tool for tuberculosis.

关键词

CRISPR, Cas12b, Mycobacterium tuberculosis, cross-priming amplification, nucleic acid detection