Products/Services Used | Details | Operation |
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Gene Services | Plasmid-bearing VSV antigenome was synthesized at GenScript (Nanjing, China), containing unique restriction sites for foreign gene expression: 5′ flanked by the T7 bacteriophage promoter, 3′ flanked by hepatitis delta virus ribozyme (HDVRz), and the T7 terminator sequence. | Get A Quote |
Hantaan virus (HTNV) is the etiological pathogen of hemorrhagic fever with renal syndrome in East Asia. There are currently no effective therapeutics approved for HTNV and other hantavirus infections. We found that griffithsin (GRFT), an algae-derived lectin with broad-spectrum antiviral activity against various enveloped viruses, can inhibit the growth and spread of HTNV. In vitro experiments using recombinant vesicular stomatitis virus (rVSV) with HTNV glycoproteins as a model revealed that the GRFT inhibited the entry of rVSV-HTNV-G into host cells. In addition, we demonstrated that GRFT prevented authentic HTNV infection in vitro by binding to the viral N-glycans. In vivo experiments showed that GRFT partia... More