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GenParts™ DNA Fragments | … DNA fragments of hUNG, hTDG, eUNG, rXRCC1, eA1, eAID, A3Gctd, A3G5.13, and A3G5.14 were synthesized and cloned into rA1-nCas9 architecture by Genscript Biotech (Nanjing … | Get A Quote |
CRISPR-guided DNA base editors (BEs) are potent genome editing tools in biotechnology and medicine. However, conventional cytosine and adenine BEs can only induce base transitions (C-to-T and A-to-G) and cannot induce base transversions. Recently, several C-to-G base editors (CGBEs) were generated and applied in human cells. By comparing them, we found that engineered deaminases rather than additional base excision repair proteins significantly improved the C-to-G efficiency. In addition, significant increase in C-to-G transversions in the GC context were determined by using rationally engineered eAID deaminase. The genome-targeting scope of CGBEs were further expanded by using SpRY Cas9 variant, which then suc... More