unassigned: To analyze the association between gene polymorphism/expression and susceptibility/prognosis of hepatocellular carcinoma (HCC).
unassigned: Peripheral venous blood from 204 hepatocellular carcinoma patients (HCC group), 242 chronic hepatitis B patients (CHB group), 215 liver cirrhosis patients (LC group) and 201 healthy volunteers (normal group) were collected. gene (including the rs9808232 locus) was amplified by PCR and the products were sequenced. Tumor tissues from patients with HCC and liver tissues from CHB, LC and normal groups were collected, and mRNA and protein expression of gene in liver tissues were tested by RT-qPCR and immunofluorescence, respectively.
unassigned: Compared with the ... More
unassigned: To analyze the association between gene polymorphism/expression and susceptibility/prognosis of hepatocellular carcinoma (HCC).
unassigned: Peripheral venous blood from 204 hepatocellular carcinoma patients (HCC group), 242 chronic hepatitis B patients (CHB group), 215 liver cirrhosis patients (LC group) and 201 healthy volunteers (normal group) were collected. gene (including the rs9808232 locus) was amplified by PCR and the products were sequenced. Tumor tissues from patients with HCC and liver tissues from CHB, LC and normal groups were collected, and mRNA and protein expression of gene in liver tissues were tested by RT-qPCR and immunofluorescence, respectively.
unassigned: Compared with the normal group, the mRNA and protein expression levels of in the HCC group increased significantly ( < 0.05), but there are no obvious increases in the CHB and LC groups ( > 0.05). gene rs9808232 polymorphism was associated with an increased susceptibility of HCC, and genotypes AC and CC may be risk factors for HCC ( < 0.001). There was a significant association between the gene rs9808232 polymorphism and the route of metastasis in HCC patients ( < 0.05). According to the Kaplan-Meier analysis, the overall survival of patients with AC and CC genotypes of rs9808232 was much shorter than that of patients with AA genotype (median overall survival, 25.5 months vs 16.0 months; Log rank = 0.006). The overall survival time of patients with high ROCK2 expression is much shorter than that of patients with low ROCK2 expression (median overall survival time, 15.0 months vs 20.5 months; Log rank = 0.008). Compared with genotype AA, the ROCK2 protein levels of genotypes AC and CC were distinctly increased in the HCC group, especially genotype CC (< 0.05).
unassigned: gene rs9808232 polymorphism may contribute to its expression in liver tissue, which may increase the susceptibility and poor prognosis of HCC.
