Products/Services Used | Details | Operation |
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GenParts™ DNA Fragments | a nucleic acid fragment encoding the full‐length human LIF with a poly His‐tag at the C terminus was synthesized and inserted in pcDNA3.1(+) vector at NotI/XbaI site by GenScript USA, Inc | Get A Quote |
In the course of our studies aiming to discover vascular bed-specific endothelial cell (EC) mitogens, we identified leukemia inhibitory factor (LIF) as a mitogen for bovine choroidal EC (BCE), although LIF has been mainly characterized as an EC growth inhibitor and an anti-angiogenic molecule. LIF stimulated growth of BCE while it inhibited, as previously reported, bovine aortic EC (BAE) growth. The JAK-STAT3 pathway mediated LIF actions in both BCE and BAE cells, but a caspase-independent proapoptotic signal mediated by cathepsins was triggered in BAE but not in BCE. LIF administration directly promoted activation of STAT3 and increased blood vessel density in mouse eyes. LIF also had protective effects on the... More