Deoxynivalenol (DON) is one of the most widespread trichothecene mycotoxins in contaminated cereal products. DON plays a vital role in the pathogenesis of , but the molecular mechanisms of DON underlying -wheat interactions are not yet well understood. In this study, a novel wheat ADP-ribosylation factor-like protein 6-interacting protein 4 gene, was identified from DON-treated wheat suspension cells by suppression subtractive hybridization (SSH). The qRT-PCR result suggested that expression is specifically activated by DON in both the intermediate susceptible wheat cultivar Zhengmai9023 and the resistant cultivar Sumai3. The transient expression results of the TaARL6IP4::GFP fusion protein indicate that e... More
Deoxynivalenol (DON) is one of the most widespread trichothecene mycotoxins in contaminated cereal products. DON plays a vital role in the pathogenesis of , but the molecular mechanisms of DON underlying -wheat interactions are not yet well understood. In this study, a novel wheat ADP-ribosylation factor-like protein 6-interacting protein 4 gene, was identified from DON-treated wheat suspension cells by suppression subtractive hybridization (SSH). The qRT-PCR result suggested that expression is specifically activated by DON in both the intermediate susceptible wheat cultivar Zhengmai9023 and the resistant cultivar Sumai3. The transient expression results of the TaARL6IP4::GFP fusion protein indicate that encodes a plasma membrane and nucleus-localized protein. Multiple sequence alignment using microscale thermophoresis showed that TaARL6IP4 comprises a conserved DON binding motif, HXXXG, and exhibits DON affinity with a dissociation constant () of 91 ± 2.6 µM. Moreover, TaARL6IP4 exhibited antifungal activity with IC values of 22 ± 1.5 µM and 25 ± 2.6 µM against and , respectively. Furthermore, TaArl6ip4 interacted with the plasma membrane of spores, resulting in membrane disruption and the leakage of cytoplasmic materials. The heterologous over-expression of conferred greater DON tolerance and resistance in Finally, we describe a novel DON-induced wheat gene, , exhibiting antifungal function and DON affinity that may play a key role in wheat interactions.
