Products/Services Used | Details | Operation |
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GenParts™ DNA Fragments | To generate pLIB-vHM-RNF219-C, human RNF219 encoding residues 434-726 was PCR amplified with primers EV0625/EV0626 from a synthetic gene fragment of RNF219 codon optimized for expression in S. frugiperda (GenScript) and | Get A Quote |
The CCR4-NOT complex acts as a central player in the control of mRNA turnover and mediates accelerated mRNA degradation upon HDAC inhibition. Here, we explored acetylation-induced changes in the composition of the CCR4-NOT complex by purification of the endogenously tagged scaffold subunit NOT1 and identified RNF219 as an acetylation-regulated cofactor. We demonstrate that RNF219 is an active RING-type E3 ligase which stably associates with CCR4-NOT via NOT9 through a short linear motif (SLiM) embedded within the C-terminal low-complexity region of RNF219. By using a reconstituted six-subunit human CCR4-NOT complex, we demonstrate that RNF219 inhibits deadenylation through the direct interaction of the α-helic... More