Products/Services Used | Details | Operation |
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Recombinant Antibody Expression | For cell culture and in vitro experiments, recombinant CPB was designed as N-terminally His-Tagged protein (Supplementary Table S1) and the codon optimized gene was ordered from GenScript® (Piscataway, NJ, USA) | Get A Quote |
Beta toxin (CPB) is a small hemolysin beta pore-forming toxin (β-PFT) produced by type C. It plays a central role in the pathogenesis of necro-hemorrhagic enteritis in young animals and humans via targeting intestinal endothelial cells. We recently identified the membrane protein CD31 (PECAM-1) as the receptor for CPB on mouse endothelial cells. We now assess the role of CD31 in CPB cytotoxicity against human endothelial and monocytic cells using a CRISPR/Cas9 gene knockout and an antibody blocking approach. CD31 knockout human endothelial and monocytic cells were resistant to CPB and CPB oligomers only formed in CD31-expressing cells. CD31 knockout endothelial and monocytic cells could be selectively enriche... More