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Mutant Libraries | Mutants of CLIMP63, p180s and KTN1 were generated in a pcDNA3.1(+) vector with a C-terminal HA-epitope tag. Specifically, Ser-to-Glu mutants (S3E, S17E, S19E) of CLIMP63 were synthesized by GenScript. | Get A Quote |
Organelles move along differentially modified microtubules to establish and maintain their proper distributions and functions. However, how cells interpret these post-translational microtubule modification codes to selectively regulate organelle positioning remains largely unknown. The endoplasmic reticulum (ER) is an interconnected network of diverse morphologies that extends promiscuously throughout the cytoplasm, forming abundant contacts with other organelles. Dysregulation of endoplasmic reticulum morphology is tightly linked to neurologic disorders and cancer. Here we demonstrate that three membrane-bound endoplasmic reticulum proteins preferentially interact with different microtubule populations, with ... More