Products/Services Used | Details | Operation |
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Recombinant Antibody Expression | A synthetic codon‐optimized gene for recombinant CI repressor protein with an N‐terminal His‐tag and TEV cleavage site (ɸ13 rCI) was purchased from Genscript (Figure (FigureA1)A1) in a pET30a(+) expression vector. The expression vector was transformed into BL21 (DE3) E. | Get A Quote |
Temperate phages are bacterial viruses that after infection either reside integrated into a bacterial genome as prophages forming lysogens or multiply in a lytic lifecycle. The decision between lifestyles is determined by a switch involving a phage-encoded repressor, CI, and a promoter region from which lytic and lysogenic genes are divergently transcribed. Here, we investigate the switch of phage ɸ13 from the human pathogen Staphylococcus aureus. ɸ13 encodes several virulence factors and is prevalent in S. aureus strains colonizing humans. We show that the ɸ13 switch harbors a cI gene, a predicted mor (modulator of repression) gene, and three high-affinity operator sites binding CI. To quantify the decisi... More