Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction | … , 3CLpro, sequence was codon optimized for expression in Escherichia coli cells and synthesized in the pET-28a(+) vector (kanamycin resistant) with a C-terminal His-tag (Genscript). … | Get A Quote |
Cold water benthic environments are a prolific source of structurally diverse molecules with a range of bioactivities against human disease. Specimens of a previously chemically unexplored soft coral, , were collected during a deep-sea cruise that sampled marine invertebrates along the Irish continental margin in 2018. Tuaimenal A (), a cyclized merosesquiterpenoid representing a new carbon scaffold with a highly substituted chromene core, was discovered through exploration of the soft coral secondary metabolome via NMR-guided fractionation. The absolute configuration was determined through vibrational circular dichroism. Functional biochemical assays and docking experiments found tuaimenal A selectively inhib... More