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Mitochondrial Localization Signal of Porcine Circovirus Type 2 Capsid Protein Plays a Critical Role in Cap-Induced Apoptosis

Vet Sci. 2021-11; 
Wanting Yu, Yuao Sun, Qing He, Chaoying Sun, Tian Dong, Luhua Zhang, Yang Zhan, Naidong Wang, Yi Yang, Yujie Sun
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Custom Vector Construction … The pcDNA3.1-EGFP vector was purchased from Addgene, and the PCV2 Cap gene (GenBank accession number: MK281580) was synthesized by GenScript (Nanjing, China) … Get A Quote

摘要

Porcine circovirus 2 (PCV2), considered one of the most globally important porcine pathogens, causes postweaning multisystemic wasting syndrome (PMWS). This virus is localized in the mitochondria in pigs with PMWS. Here, we identified, for the first time, a mitochondrial localization signal (MLS) in the PCV2 capsid protein (Cap) at the N-terminus. PK-15 cells showed colocalization of the MLS-EGFP fusion protein with mitochondria. Since the PCV2 Cap also contained a nuclear localization signal (NLS) that mediated entry into the nucleus, we inferred that the subcellular localization of the PCV2 Cap is inherently complex and dependent on the viral life cycle. Furthermore, we also determined that deletion of the ML... More

关键词

Cap, MLS, PCV2, subcellular localization