| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | … The operon was synthetized and cloned using SacI and SpeI restriction sites by GenScript yielding … Nevertheless, the MS analysis allows us to discard that new methylation might have … | Get A Quote |
Pederin is a potent polyketide toxin that causes severe skin lesions in humans after contact with insects of genus Due to its potent anticancer activities, pederin family compounds have raised the interest of pharmaceutical industry. Despite the extensive studies on the cluster of biosynthetic genes responsible for the production of pederin, it has not yet been possible to isolate and cultivate its bacterial endosymbiont producer. However, the marine bacterium sp. PHM005 was recently reported to produce labrenzin, the closest pederin analog. By cloning a synthetic gene encoding one of the three -methyltraferase of the pederin cluster into sp. PHM005 we have been able to produce pederin for the first time by... More
