Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning | … All constructs were synthesized and subcloned by Genscript. The SFSp synthesis is … into the lentiviral transfer plasmids by Genscript following our subcloning methodology (Truong et al. … | Get A Quote |
For clinical cell-based therapies (e.g. CAR-T cells), the genomic integration of therapeutic genes into cells are selected using inefficient resistance genes of host origin to avoid potential immune response from using more efficient resistance genes of foreign origin. In principle, a serum-responsive promoter could express efficient resistance genes during the cell manufacturing stage that could then diminish during in vivo administration. To avoid genomic instability, we designed a synthesis-friendly serum-responsive promoter (SFSp) with no extreme GC ratios or repeats greater than 9 base pairs. SFSp was used to express a fluorescent reporter, whose expression was diminished after serum starvation. Furthermor... More