Products/Services Used | Details | Operation |
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Plasmid DNA Preparation | … coli plasmid expression vectors (GenScript, N-terminal 8× His-tagged followed by a TEV cleavage site). For all the designed proteins, the sequence of the N-terminal tag used is … | Get A Quote |
Engineered proteins generally must possess a stable structure in order to achieve their designed function. Stable designs, however, are astronomically rare within the space of all possible amino acid sequences. As a consequence, many designs must be tested computationally and experimentally in order to find stable ones, which is expensive in terms of time and resources. Here we use a high-throughput, low-fidelity assay to experimentally evaluate the stability of approximately 200,000 novel proteins. These include a wide range of sequence perturbations, providing a baseline for future work in the field. We build a neural network model that predicts protein stability given only sequences of amino acids, and compa... More