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Identifying Function Determining Residues in Neuroimmune Semaphorin 4A

Int J Mol Sci. 2022-03; 
Svetlana P Chapoval, Mariah Lee, Aaron Lemmer, Oluwaseyi Ajayi, Xiulan Qi, Andrew F Neuwald, Achsah D Keegan
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Mutant Libraries … domain is located in the N-terminus of semaphorin molecules … = 66kDa was purchased from GenScript. Mutant Sema4A … ) and were prepared by GenScript. The mutant proteins were … Get A Quote

摘要

Semaphorin 4A (Sema4A) exerts a stabilizing effect on human Treg cells in PBMC and CD4+ T cell cultures by engaging Plexin B1. Sema4A deficient mice display enhanced allergic airway inflammation accompanied by fewer Treg cells, while Sema4D deficient mice displayed reduced inflammation and increased Treg cell numbers even though both Sema4 subfamily members engage Plexin B1. The main objectives of this study were: 1. To compare the in vitro effects of Sema4A and Sema4D proteins on human Treg cells; and 2. To identify function-determining residues in Sema4A critical for binding to Plexin B1 based on Sema4D homology modeling. We report here that Sema4A and Sema4D display opposite effects on human Treg cells in in... More

关键词

Plexin B1, Semaphorin 4A, human Treg cells, immunotherapeutics for asthma, mutated proteins