| Products/Services Used | Details | Operation |
|---|---|---|
| Mutant Libraries | … aureus was purchased from GenScript. Escherichia coli BL21(DE3) cells (Invitrogen) were … The BrxA C56S mutant plasmid was purchased from GenScript and the protein was … | Get A Quote |
Bacilliredoxins are small proteins that are involved in redox homeostasis in bacillithiol-producing bacteria. They reduce mixed bacillithiol disulfides on protected proteins through a disulfide-exchange reaction, restoring the thiol group on the target protein. Bacilliredoxins contain an unusual conserved CGC motif, and their exact catalytic mechanism remains unclear. Here, a 1.6 Å resolution X-ray crystallographic structure of the bacilliredoxin BrxA (YphP) from Staphylococcus aureus is presented. The structure contains bacillithiol in a mixed disulfide with Cys54, as well as a disulfide linkage at Cys56, which may play a role in dimer stabilization. The structure presented here will provide insight into th... More
