GenScript, USA) was used according to the manufacturer's protocol, using Tris-Mops-SDS running buffer in a Mini-Protean 3 electrophoresis cell (Bio-Rad Laboratories Richmond, CA, USA). ...">
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Influence of N-Glycosylation on the Morphogenesis and Growth of Paracoccidioides brasiliensis and on the Biological Activities of Yeast Proteins.

PLoS One.. 2011-12;  6(12):e29216
Dos Reis Almeida FB, Carvalho FC, Mariano VS, Alegre AC, Silva Rdo N, Hanna ES, Roque-Barreira MC. Departamento de Biologia Celular e Molecular e Bioagentes PatogÊnicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil
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摘要

The fungus Paracoccidioides brasiliensis is a human pathogen that causes paracoccidioidomycosis, the most prevalent systemic mycosis in Latin America. The cell wall of P. brasiliensis is a network of glycoproteins and polysaccharides, such as chitin, that perform several functions. N-linked glycans are involved in glycoprotein folding, intracellular transport, secretion, and protection from proteolytic degradation. Here, we report the effects of tunicamycin (TM)-mediated inhibition of N-linked glycosylation on P. brasiliensis yeast cells. The underglycosylated yeasts were smaller than their fully glycosylated counterparts and exhibited a drastic reduction of cell budding, reflecting impairment of growth and mor... More

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