Products/Services Used | Details | Operation |
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Plasmid DNA Preparation | … assembly using PCR reactions that span each inter-chunk junction. Identical PCR results were … were synthesized and cloned into a plasmid vector (GenScript). For the neo-chromosome … | Get A Quote |
The Synthetic Yeast Genome Project (Sc2.0) represents the first foray into eukaryotic genome engineering and a framework for designing and building the next generation of industrial microbes. However, the laboratory strain S288c used lacks many of the genes that provide phenotypic diversity to industrial and environmental isolates. To address this shortcoming, we have designed and constructed a neo-chromosome that contains many of these diverse pan-genomic elements and which is compatible with the Sc2.0 design and test framework. The presence of this neo-chromosome provides phenotypic plasticity to the Sc2.0 parent strain, including expanding the range of utilizable carbon sources. We also demonstrate that the ... More