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Gene Promotes Muscle Growth and Development in Meat Ducks

Genes (Basel). 2022-09; 
Tingting Zhou, Yijing Wu, Yulin Bi, Hao Bai, Yong Jiang, Guohong Chen, Guobin Chang, Zhixiu Wang
Products/Services Used Details Operation
DNA Sequencing … Using GenScript protein preparation gel, SDS-PAGE vertical electrophoresis and … and sequencing (performed by Nanjing Qingke Biotechnology Co., Ltd., Nanjing, China) and the … Get A Quote

摘要

To explore the effect of in the muscle growth and development of meat ducks, single-nucleotide polymorphism loci were screened at the DNA level in the meat duck population with highest and lowest feed conversion rates. The expression of was detected using reverse-transcription quantitative polymerase chain reaction. The protein expression of MYOZ1 was detected using Western blotting at the protein level. The results showed that there was a base mutation site at 30 bp and 158 bp in the fourth exon of , which was mutated from C to T (exon4 C30T) and from G to A (exon4 G158A), respectively. The allele frequency of the locus was significantly different between the high and low feed conversion rate groups ( < ... More

关键词

MYOZ1, feed conversion ratio, meat duck, muscle growth and development, protein expression, single nucleotide polymorphism