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A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasma

PLoS ONE. 2021-01; 
Shreyas Jadhav, Sudipta Tripathi, Anil Chandrekar, Sushrut S Waikar, Li-Li Hsiao
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Gene Synthesis … The plate was then processed as above. secKL Ab generation. Following peptide sequence was provided to GenScript (GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854) for antibody generation: SQLTKPISSLTKPYH … Get A Quote

摘要

αKlotho is primarily known to express as a transmembrane protein. Proteolytic cleavage results in shedding of the extracellular domain which enters systemic circulation. A truncated form of αKlotho resulting from alternative splicing of the αKLOTHO transcript exists and is believed to be secreted, thereby also entering systemic circulation. Existing ELISA methods fail to distinguish between the two circulating isoforms resulting in inconsistencies in assessing circulating αKlotho levels. We have exploited a unique 15aa peptide sequence present in the alternatively spliced secreted isoform to generate an antibody and show that it is able to specifically detect only the secreted Klotho isoform in human plasma... More

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