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NMR Studies of the Interaction of Calmodulin with IQ Motif Peptides.

Methods Mol Biol.. 2013-04;  963:173-186
Damo SM, Feldkamp MD, Chagot B, Chazin WJ. Departments of Biochemistry and Chemistry, Vanderbilt University, Nashville, TN, USA
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摘要

Calmodulin (CaM) is a ubiquitous EF-hand calcium sensor protein that transduces calcium signals in a wide range of signaling pathways. Structural analysis of complexes with peptides has provided valuable insights into the remarkable variety in the way in which CaM interacts with and activates its targets. Among these various targets, CaM has been shown to be an essential component of a calcium-sensing regulatory apparatus for a number of voltage-gated ion channels. NMR spectroscopy has proven to be a powerful tool for the structural characterization of CaM-peptide complexes, in particular for the study of IQ motifs, which bind CaM at the basal level of calcium in cells and thereby serve to localize CaM to its s... More

关键词

Calmodulin; NaV1.5; IQ motif; 15N-1H HSQC; NMR spectroscopy; Protein complex; Co-expression; Calcium binding protein; Calcium signaling; EF-hand