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BIM induction of apoptosis triggered by EGFR-sensitive and resistance cell lines of non-small-cell lung cancer.

Med Oncol.. 2011-06;  28(2):572-577
Li Z, Zhou S, Zhang L, Su C, Hang J, Zhao Y, Su B, Zhou C. Department of Oncology; Pulmonary Hospital, Tongji University School of Medicine, Yangpu Region, Shanghai, 200433, China.
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摘要

We sought to improve the understanding of oncogene-dependent and independent non-small-cell lung cancer (NSCLC), which could provide insight into mechanism of sensitivity and/or resistance to tyrosine kinase inhibitors or chemotherapeutics. NSCLC cell lines with different EGFR genotypes were used in this study; MTT assay and flow cytometry were applied to study the sensitivities of these cell lines to gefitinib and cisplatin. Western blot was performed to determine the expression levels of BIM and other Bcl-2 family proteins pre- and pro-treatment. Gefitinib provoked apoptosis of caspase activation via the intrinsic pathways and significantly up-regulated expression of BIM protein in drug-sensitive PC-9 cell li... More

关键词

Non-small-cell lung cancer; EGFR; BIM; Apoptosis