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Specific inhibition of AQP1 water channels in human pulmonary microvascular endothelial cells by small interfering RNAs.

J Trauma Acute Care Surg.. 2012-01;  72(1):150-61
C Gao, J Tang, R Li, J Huan. Department of Burn and Plastic Surgery, Ninth People's Hospital, Rui Jin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
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摘要

BACKGROUND: Aquaporin (AQP)-1 is expressed in most microvasculature endothelial cells forming water channels that play major roles in a variety of physiologic processes. Our aim was to investigate the regulatory functions of AQP1 on trancellular and paracellular permeability. METHODS: We designed, synthesized, and used small interfering RNAs (siRNAs) selective for AQP1 and investigated their effectiveness in altering AQP1-mediated permeability in human pulmonary microvascular endothelial cells. RESULTS: Twenty-four hours after transfection of ECs with siRNAs targeting two different regions of the AQP1 transcript, AQP1 protein was inhibited by 47.8% to 74.6%. siRNAs containing the same percent of base pairs as... More

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