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| Proteins, Expression, Isolation and Analysis | … The eluent was then loaded to anti-FLAG G1 affinity resin (GenScript). The resin was washed with buffer containing 25 mM HEPES-Na, pH 7.0, 400 mM NaCl, 0.015% GDN, 1 mM EDTA and 2 mM PMSF. The protein was then eluted with buffer containing 25 mM HEPES-Na, pH … | Get A Quote |
The cation channel of sperm (CatSper) is essential for sperm motility and fertility. CatSper comprises the pore-forming proteins CATSPER1-4 and multiple auxiliary subunits, including CATSPERβ, γ, δ, ε, ζ, and EFCAB9. Here we report the cryo-electron microscopy (cryo-EM) structure of the CatSper complex isolated from mouse sperm. In the extracellular view, CATSPER1-4 conform to the conventional domain-swapped voltage-gated ion channel fold, following a counterclockwise arrangement. The auxiliary subunits CATSPERβ, γ, δ and ε-each of which contains a single transmembrane segment and a large extracellular domain-constitute a pavilion-like structure that stabilizes the entire complex through interactions w... More
