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Custom Vector Construction | … Full-length murine-beta-catenin (UniProt ID Q02284, 342 purchased from GenScript) was transferred to a K151 vector system using transfer- 343 PCR. Both proteins start with an N-terminal His12-tag followed by the Sumo protein. 344 345 … | Get A Quote |
Intrinsically disordered proteins often form dynamic complexes with their ligands. Yet, the speed and amplitude of these motions are hidden in classical binding kinetics. Here, we directly measure the dynamics in an exceptionally mobile, high-affinity complex. We show that the disordered tail of the cell adhesion protein E-cadherin dynamically samples a large surface area of the protooncogene β-catenin. Single-molecule experiments and molecular simulations resolve these motions with high resolution in space and time. Contacts break and form within hundreds of microseconds without a dissociation of the complex. The energy landscape of this complex is rugged with many small barriers (3 to 4 ) and reconciles sp... More