| Products/Services Used | Details | Operation |
|---|---|---|
| GenParts™ DNA Fragments | … Four overlapping synthetic DNA fragments were designed in silico and supplied by the manufacturer (Genscript manufacturers). The first and last fragments were directly flanked at their 5′ and 3′extremities by the human cytomegalovirus promoter (pCMV) and the hepatitis … | Get A Quote |
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most challenging infectious disease of pig populations causing devastating economic loss to swine industry. Reverse genetics allow to engineer modified viruses such attenuated strains for vaccine development. Some reverse genetic systems were described for PRRSVs but, due to genome complexity of PRRSVs, construction and modification of such systems remain laborious and time-consuming. In this study, we described a reverse genetics approach based on the "Infectious-Subgenomic Amplicons" (ISA) method to rescue infectious PRRSV particles. Permissive cells were transfected with 4 overlapping synthetic DNA fragments covering the entire genome ... More
