| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Antibody Expression | … RNA probes were synthesized and labeled with 6-FAM at the 3' end by GenScript (Nanjing, China). For REMSAs, the method was similar to a previously described protocol with little modification (Xiao et al., 2018). The recombinant protein was incubated with a labeled RNA … | Get A Quote |
Chloroplast biogenesis and development are highly complex processes requiring interactions between plastids and nuclear genomic products. Pentatricopeptide repeat (PPR) proteins play an essential role in the development of chloroplasts; however, it remains unclear how RNA editing factors influence soybean development. In this study, a mutant () was identified with decreased chlorophyll contents. Genetic mapping revealed that a single-nucleotide deletion at position 1949 bp in the gene in the mutant, resulting in a truncated GmPGL2 protein. The nuclear-encoded is a PLS-type PPR protein that localizes to the chloroplasts. The C-to-U editing efficiencies of , , , , , and were reduced in the mutant. RNA elect... More
