Products/Services Used | Details | Operation |
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Catalog Peptides | … The N-terminal tag (6xHis tag – 28 aa tag – GGGGS linker) was cleaved with His tagged bovine enterokinase as per the manufacturers instructions (GenScript Biotech, USA). Please note that tagged peptides were designed with the enterokinase cleavage site (highly specific … | Get A Quote |
West Nile virus (WNV), re-emerging neurotropic flavivirus, can cross the blood-brain barrier (BBB) and cause fatal encephalitis and meningitis. Infection of the human brain microvascular endothelial cells (hBMECs), building blocks of the BBB, represents the pivotal step in neuroinvasion. Domain III (DIII) of the envelope (E) glycoprotein is a key receptor-binding domain, thus, it is an attractive target for anti-flavivirus strategies. Here, two combinatorial phage display peptide libraries, Ph.D.-C7C and Ph.D.-12, were panned against receptor-binding site (RBS) on DIII to isolate peptides that could block DIII. From series of pannings, nine peptides (seven 7-mer cyclic and two 12-mer linear) were selected and o... More