Products/Services Used | Details | Operation |
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Catalog Peptides | … The substrate peptides (Genscript, described below) used in the assay corresponded either to one of three peptides: the first 7 residues of Htt followed by a positively charged poly-Arginine tag for electrostatic capture by the phosphocellulose papers used in the assays, the first … | Get A Quote |
Huntington's disease (HD) is a neurodegenerative disorder caused by a poly-CAG expansion in the first exon of the HTT gene, resulting in an extended poly-glutamine tract in the N-terminal domain of the Huntingtin (Htt) protein product. Proteolytic fragments of the poly-glutamine-containing N-terminal domain form intranuclear aggregates that are correlated with HD. Post-translational modification of Htt has been shown to alter its function and aggregation properties. However, the effect of N-terminal Htt acetylation has not yet been considered. Here, we developed a bacterial system to produce unmodified or N-terminally acetylated and aggregation-inducible Htt protein. We used this system together with biochemica... More