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Custom Vector Construction | … The C-terminal domain of PLIN1 encoding residues from 401 to 522 with 6xHis tag was synthesized (GenScript, Netherlands) and cloned into pET24a vector and expressed in E. coli strain Rosetta (DE3, Novagen) by induction with 1 mM IPTG at 30 C. Harvested cells were … | Get A Quote |
Aquaporins play a crucial role in water homeostasis in the human body, and recently the physiological importance of aquaporins as glycerol channels have been demonstrated. The aquaglyceroporins (AQP3, AQP7, AQP9 and AQP10) represent key glycerol channels, enabling glycerol flux across the membranes of cells. Adipocytes are the major source of glycerol and during lipolysis, glycerol is released to be metabolized by other tissues through a well-orchestrated process. Here we show that both AQP3 and AQP7 bind to the lipid droplet protein perilipin 1 (PLIN1), suggesting that PLIN1 is involved in the coordination of the subcellular translocation of aquaglyceroporins in human adipocytes. Moreover, in addition to aquag... More