Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction | … used as a backbone for mammalian expression plasmids and pUC57simple (GenScript) was used as a backbone for pox transfer vectors. … K1L-FLAG-IN was verified by western blot analysis and immunofluorescence using the FLAG-tag. RK13 cells with stable expression of K1L-… | Get A Quote |
Attenuated vaccine strains of lumpy skin disease virus (LSDV) have become increasingly popular as recombinant vaccine vectors, to target both LSDV, as well as other pathogens, including human infectious agents. Historically, these vaccine strains and recombinants were generated in primary (lamb) testis (LT) cells, Madin-Darby bovine kidney (MDBK) cells or in eggs. Growth in eggs is a laborious process, the use of primary cells has the potential to introduce pathogens and MDBK cells are known to harbor bovine viral diarrhea virus (BVDV). In this study, data is presented to show the growth of an attenuated LSDV strain in baby hamster kidney (BHK-21) cells. Subsequently, a recombinant LSDV vaccine was generated in... More