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Custom Vector Construction | … coli synthetic genes from GenScript, (Piscataway, NJ), already cloned into pET20b( +) expression vector, containing an N-terminal His 6 -tag and a C-terminal His 6 -tag respectively. The SARS–CoV macro domain contained one artificial N-terminal residue (M-1) and six … | Get A Quote |
SARS-CoV and MERS-CoV Macro Domains (MDs) exhibit topological and conformational features that resemble the nsP3b macro (or "X") domain of SARS-CoV-2. Indeed, all the three domains (SARS-CoV-2, SARS-CoV and MERS-CoV MDs) fold in a three-layer α/β/α sandwich structure, as reported by crystallographic structural investigation of SARS-CoV MD and MERS-CoV MD. These viral MDs are able to bind ADP-ribose as many other MDs from different kingdoms. They have been characterized also as de-ADP-ribosylating enzymes. For this reason, these viral macrodomains recently emerged as important drug targets since they can counteract antiviral ADP-ribosylation mediated by poly-ADP-ribose polymerase (PARPs). Even in presence of ... More