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GABARAP sequesters the FLCN-FNIP tumor suppressor complex to couple autophagy with lysosomal biogenesis

Sci Adv. 2021-10;

Jonathan M Goodwin, Ward G Walkup, Kirsty Hooper, Taoyingnan Li, Chieko Kishi-Itakura, Aylwin Ng, Timothy Lehmberg, Archana Jha, Sravya Kommineni, Katherine Fletcher, Jorge Garcia-Fortanet, Yaya Fan, Qing Tang, Menghao Wei, Asmita Agrawal, Sagar R Budhe, Sreekanth R Rouduri, Dan Baird, Jeff Saunders, Janna Kiselar, Mark R Chance, Andrea Ballabio, Brent A Appleton, John H Brumell, Oliver Florey, Leon O Murphy

Products/Services Used	Details	Operation
Custom Vector Construction	… synthesized (GenScript, USA) and provided as entry clones. Gateway recombination was used to shuttle cassettes into pcDNA-DEST40 (Life Technologies) or a lentiviral vector allowing tetracycline-inducible expression referred to as Tet-Lenti (synthesized by GenScript, USA). …	Get A Quote