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Mutagenesis Services | … minal FLAG-tagged FCRL1 cDNA sequence containing an internal BamHI restriction site and a 3' SalI restriction site for further mutagenesis was custom-ordered from GenScript. The insert sequence was ligated into pLenti-CMV-GFP-Puro vector (gift from Eric Campeau, … | Get A Quote |
Regulation of BCR signaling has important consequences for generating effective Ab responses to pathogens and preventing production of autoreactive B cells during development. Currently defined functions of Fc receptor-like (FCRL) 1 include positive regulation of BCR-induced calcium flux, proliferation, and Ab production; however, the mechanistic basis of FCRL1 signaling and its contributions to B cell development remain undefined. Molecular characterization of FCRL1 signaling shows phosphotyrosine-dependent associations with GRB2, GRAP, SHIP-1, and SOS1, all of which can profoundly influence MAPK signaling. In contrast with previous characterizations of FCRL1 as a strictly activating receptor, we discover a ro... More