Products/Services Used | Details | Operation |
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ORF cDNA Clones/MolecularCloud | … (Genscript, Piscataway, NJ). To generate revertant viruses OV-IA82-RV113 HA (ORFV113 with HA tag) and OV-IA82-RV113 (untagged ORFV113), recombination plasmids containing ORFV113 sequence containing C-terminal HA tag or untagged ORFV113 and VV7.5-driven … | Get A Quote |
Viruses have evolved mechanisms to subvert critical cellular signaling pathways that regulate a wide range of cellular functions, including cell differentiation, proliferation and chemotaxis, and innate immune responses. Here, we describe a novel ORFV protein, ORFV113, that interacts with the G protein-coupled receptor Lysophosphatidic acid receptor 1 (LPA1). Consistent with its interaction with LPA1, ORFV113 enhances p38 kinase phosphorylation in ORFV infected cells in vitro and in vivo, and in cells transiently expressing ORFV113 or treated with soluble ORFV113. Infection of cells with virus lacking ORFV113 (OV-IA82Δ113) significantly decreased p38 phosphorylation and viral plaque size. Infection of cells wi... More