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Peptide Tools | … coli host organism, without altering the amino acid sequence of the protein, by GenScript Co., Hong Kong. The primary structure of the L-ASNase revealed conservation of the D. chrysanthemi L-ASNase active site signature, comprised of the amino acid residues Thr 16,96 , Tyr … | Get A Quote |
Microbial L-asparaginases are aminohydrolases that hydrolyze L-asparagine to L-aspartate. They are used to treat acute lymphoblastic leukemia and Hodgkin's lymphomas and in food industries. Increasing demand for L-ASNases is therefore needed. In the current study, the recombinant L-ASNase from (DcL-ASNase) was cloned into pET28a (+) expression vector and expressed in as a 6His-tagged fusion protein and purified using Ni chelated Sepharose chromatography resin, yielding a highly purified enzyme. Kinetics analysis allowed the determination of its substrate specificity and the physicochemical parameters that affect enzyme activity. The enzyme showed operational stability at 37 °C and 45 °C. The immunogenic... More