| Products/Services Used | Details | Operation |
|---|---|---|
| Plasmid DNA Preparation | … gene (Supplementary Table 1) was constructed as described previously 49 , EF-P. A pBAD-His 6 -SUMO plasmid harboring the efp gene (from Pseudomonas aeruginosa PAO1, synthesized and cloned by GenScript… Plasmids containing the gene of interest were … | Get A Quote |
Nanopores are single-molecule sensors used in nucleic acid analysis, whereas their applicability towards full protein identification has yet to be demonstrated. Here, we show that an engineered Fragaceatoxin C nanopore is capable of identifying individual proteins by measuring peptide spectra that are produced from hydrolyzed proteins. Using model proteins, we show that the spectra resulting from nanopore experiments and mass spectrometry share similar profiles, hence allowing protein fingerprinting. The intensity of individual peaks provides information on the concentration of individual peptides, indicating that this approach is quantitative. Our work shows the potential of a low-cost, portable nanopore-based... More
