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Monoclonal Antibody Services | … After the proteins were transferred onto a nitrocellulose membrane, the membrane was blocked with 4% BSA in TBS and then incubated with peroxidase-conjugated mouse monoclonal antibody against GST tag (GenScript, Nanjing). rSpSR-B2ER protein was visualized with an … | Get A Quote |
Although class B scavenger receptors (SR-Bs) in mammals are multifunctional molecules, the functions of SR-Bs in invertebrates remain largely unknown. In this study, we characterized an SR-B homolog, namely SpSR-B2, from Scylla paramamosain. SpSR-B2 shared high similarity with mammalian SR-Bs, and exhibited specific binding activity to ac-LDL, indicating that it may be a new member of SR-B class in invertebrates. SpSR-B2 was upregulated after challenge with white spot syndrome virus (WSSV) or bacteria. Binding assays showed that SpSR-B2 specifically interacted with WSSV envelope protein VP24. Besides, SpSR-B2 could bind to all tested bacterial cells and agglutinate these bacteria. SpSR-B2 also exhibited a stron... More