| Products/Services Used | Details | Operation |
|---|---|---|
| Plasmid DNA Preparation | … The full length nucleic acid sequence of TfuDyP (NCBI Accession number Q47KB1) was chemically synthesized and inserted into plasmid pET-28a (+) (GenScript Biotech Corporation, Nanjing, China). The plasmid containing the target gene was transformed into E. coli BL21(… | Get A Quote |
Wastewater containing recalcitrant dyes causes environmental problems. A new superfamily of heme-containing peroxidases, dye-decolorizing peroxidases (DyPs), has been found to decolorize different kinds of dyes, especial anthraquinone dyes efficiently. However, the mechanism of dyes degradation by DyPs has not been fully understood and the toxicity of dye degradation intermediates by DyPs catalysis to microbes is unclear. In this study, a purified recombinant Thermobifida fusca DyP (TfuDyP) in E. coli BL21(DE3) was used to treat Reactive Blue 19 (RB19), an anthraquinone dye. The reaction intermediates analyzed by ultra performance liquid chromatography/mass spectroscopy (UPLC-MS) indicated the initial site of T... More
