A novel bacterium, designated BD-1, was isolated from a sludge sample. Cells of the novel Gram-stain-negative strain were identified to be facultative anaerobic, non-motile and short rod-shaped. Growth occurred at 15-37 °C (optimum, 30 °C), pH 5.0-10.0 (pH 7.0) and in 0-4.0 % NaCl (2.0 %, w/v). The 16S rRNA gene sequence of strain BD-1 showed the highest sequence similarity to DSM 14619 (97.0 %), followed by DSM 21699 (96.3 %) and less than 96 % to other related strains. The phylogenetic trees revealed that strain BD-1 clustered within the genus . Summed feature 3 (C 7 and/or C 6, 48.2 %), C (23.2 %) and summed feature 8 (C 7 and/or C 6, 8.6 %) were the major fatty acids (>5 %), a... More
A novel bacterium, designated BD-1, was isolated from a sludge sample. Cells of the novel Gram-stain-negative strain were identified to be facultative anaerobic, non-motile and short rod-shaped. Growth occurred at 15-37 °C (optimum, 30 °C), pH 5.0-10.0 (pH 7.0) and in 0-4.0 % NaCl (2.0 %, w/v). The 16S rRNA gene sequence of strain BD-1 showed the highest sequence similarity to DSM 14619 (97.0 %), followed by DSM 21699 (96.3 %) and less than 96 % to other related strains. The phylogenetic trees revealed that strain BD-1 clustered within the genus . Summed feature 3 (C 7 and/or C 6, 48.2 %), C (23.2 %) and summed feature 8 (C 7 and/or C 6, 8.6 %) were the major fatty acids (>5 %), and ubiquinone-8 was the respiratory quinone. Phosphatidylethanolamine, phosphatidylmethylethanolamine and phosphatidylglycerol were identified as the major polar lipids. Meanwhile, the G+C content of the DNA was 63.6 mol% based on the draft genome analysis. The average nucleotide identity and digital DNA-DNA hybridization values between strain BD-1 and DSM 14619 were 74.5 and 21.4 %, respectively. In addition, the novel strain completely degraded 500 mg l phenylacetic acid within 72 h under the condition of 3 % NaCl. Given the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain BD-1 was considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The strain is a potential resource for the bioremediation of phenylacetic acid contaminated water. The type strain is BD-1 (=CGMCC 1.18541=KCTC 82183).