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CRISPR/Cas12a-assisted rapid identification of key beer spoilage bacteria

Innovative Food Science and Emerging Technologies . 2021-10;

Qingzhou Menga, Hongmei Yangb, Guiquan Zhangc, Wenjun Sund, Peixiang Mae, Xinyi Liuf, Lu Dangg, Guanglei Lid, Xingxu Huangd, Xinjie Wangh, Jianghuai Liuc, Qibin Lenga

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Synthetic sgRNA and crRNA Service	Bacteria genome DNA were extracted using a commercial kit (GenElute™ Bacterial Genomic DNA Kit, NA2100) according to the manufacturer’s instructions. The selected 16S-rDNA fragment of L. brevis, P. damnosus, L. lindneri were synthesized by GenScript (Nanjing, China) and cloned into the pUC57 vector.	Get A Quote