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Backbone chemical shift spectral assignments of SARS coronavirus-2 non-structural protein nsp9

Biomol NMR Assign. 2021-03;

Erika F Dud??s, Rita Puglisi, Sophie Marianne Korn, Caterina Alfano, Maria Laura Bellone, Fabrizio Dal Piaz, Geoff Kelly, Elisa Monaca, Andreas Schlundt, Harald Schwalbe, Annalisa Pastore

Products/Services Used	Details	Operation
Gene Synthesis	The gene was obtained from GenScript Biotech (Netherlands), inserted into the pET3b-based vector pKM263,	Get A Quote

摘要

As part of an International consortium aiming at the characterization by NMR of the proteins of the SARS-CoV-2 virus, we have obtained the virtually complete assignment of the backbone atoms of the non-structural protein nsp9. This small (12?kDa) protein is encoded by ORF1a, binds to RNA and seems to be essential for viral RNA synthesis. The crystal structures of the SARS-CoV-2 protein and other homologues suggest that the protein is dimeric as also confirmed by analytical ultracentrifugation and dynamic light scattering. Our data constitute the prerequisite for further NMR-based characterization, and provide the starting point for the identification of small molecule lead compounds that could interfere with RN... More