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Phylogenetic identification and microbial diversity in snow of the summit (8201 m) of Cho Oyu Mountain, Tibet.

Chinese Science Bulletin.. 2008-11;  53(21):3317-3323
XiaoMei Tong, Fang Chen, Jun Yu, Sang Hua, Ciren Asan, JiangBai Luosang, Wei Wang, Liang Yu, XiaoGuang Zheng, Jian Wang. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 101300, China
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摘要

The bacterial diversity and abundance in snow of the summit (8201 m) of Cho Oyu mountain, Tibet, were analyzed by 16S rRNA gene sequencing followed by scanning electronic microscopy analysis. Most of bacteria were found to be of spherical or oval shape (>95%). Bacterial 16S rDNA sequences were classified into 5 genera (Caulobacter, Ralstonia, Cupriavidus, Pelomonas and Pseudomonas). Gammaproteobacteria were the most abundant (91.25%) among the library that consists of 594 clones. The sequences found in this study are highly similar to those previously retrieved from other cold environments, such as ice core, sea ice, permafrost and snow. The results showed that the cold and barren environments strongly influ... More

关键词

Cho Oyu; snow; 16S rRNA gene; phylogenetic tree