| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … Restriction enzymes, T4 DNA ligase, and exTaq DNA polymerase were purchased from Takara Bio Corporation (Dalian, China). The protein molecular weight marker was purchased from Nanjing Genscript Corporation (Nanjing, China) … | Get A Quote |
Information concerning food composition, including information on its glucose content, is essential for modern food industry due to greater consumer awareness and expectations. In this work, the gene encoding d-glucose dehydrogenase (GDH) from Bacillus Natto was expressed in Escherichia coli BL21(DE3) firstly. Ni-IDA column was used for the purification of GDH. Then, the purified GDH was used to construct a color system with stable and effective measurement of concentration of d-glucose. The smart phone photographing and the software Microsoft Photoshop have been used in the system for determination of the color. The enzymatic analysis system can detect the concentration of d-glucose from 5 mM to 40 mM, and o... More
