| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … The sequence of the hsa-miR-4454 mature body is 5′-GGAUCCGAGUCACGGCA CCA-3′ (http://www.mirbase.org/cgi-bin/mature.pl?mature_acc=MIMAT0018976), which was synthesized by GenScript (Nanjing) Co., Ltd. (Nanjing, Jiangsu, China) … | Get A Quote |
The abnormal expression of HPV16 E6/E7 activates oncogenes and/or inactivates tumor suppressor genes, resulting in the selective growth and malignant transformation of cancer cells. miR-4454 was selected by sequencing due to its abnormal high expression in HPV16 E6/E7 positive CaSki cell compared with HPV16 E6/E7 negative C33A cell. Overexpression of miR-4454 enhances cervical cancer cell invasion and migration. ABHD2 and NUDT21 are identified as a target gene of miR-4454.The effects of ABHD2 and NUDT21 on migration and invasion of CaSki and C33A cells were determined. The dual luciferase and RT-qPCR assays confirmed that miR-4454 might regulate its targets ABHD2 and NUDT21 to promote the proliferation, invasio... More
